View research View latest news Sign up for updates. Both organisms were grown on a light-dark cycle, with the light period starting at Strong diel patterns in these three aspects of carbon fixation occurred in both organisms, with maximal levels in the light period and minima in the dark.
MATERIALS AND METHODS
DNase I footprint analyses indicated that R. A cosmid carrying the R. DNase I footprint analyses, using R. Expression from promoter insertion mutants showed that the function of the promoter distal regulatory region was helical phase-dependent.
A pilot field experiment to assess the relationship between traditional biogeochemical rate measurements and transcriptional activity of microbial populations was carried out at the LEO 15 site off Tuckerton, N. Similar diel patterns of carbon fixation and rbcL gene expression were observed in three of four time series, with maxima for photosynthetic capacity P max and rbcL mRNA occurring between 10 a. The lowest P max and rbcL levels were detected between 6 p. Clone libraries from reverse transcription-PCR-amplified rbcL mRNA indicated the presence of several chromophytic algae diatoms, prymnesiophytes, and chrysophytes and some eukaryotic green flagellates. Analogous results were obtained from amplified small rRNA sequences and secondary pigment analysis.
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